Table 2.
Comparision of culture duration, total consumed glucose and glutamine, produced lactatemax, ammoniamax and C12 antibody, and cell density (Xmax) in batch, fed-batch, and perfusion culture processes
| Culture process |
Culture duration h |
Total Gluc g |
Total Glun g |
Lacmax mmol/L |
Ammmax mmol/L |
C12 Ab amount mg |
Xmax 105cells/mL |
Broth volume L |
|---|---|---|---|---|---|---|---|---|
| Batch | 144 | 5.97 | 1.095 | 17.76 | 3.6 | 377.64 | 39.6 | 2.0 |
| Fed-batch A | 270 | 29.80 | 2.399 | 24 | 5.2 | 1,020.94 | 48.2 | 4.0 |
| Fed-batch B | 294 | 25.67 | 2.093 | 26.4 | 5.5 | 1,128.40 | 54.7 | 4.0 |
| Perfusion | 408 | 41.46 | 2.57 | 15.15 | 3.8 | 1,854.00 | 197.5 | 12.0 |