Table 3.

Comparision of Y_Vx/Gluc, Y_Vx/Gln,Y_Lac/Gluc,Y_Amm/Gln,Y_Ab/Vx,Y_Ab/Gluc, and Y_Ab/Gln in batch, fed-batch, perfusion culture processes

Culture process	YVx/Gluc 108cells/g	YVx/Glun 108cells/g	Ylac/Gluc g/g	YAmm/Glun mg/g	YAb/Vx mg/105cells	YAb/gluc mg/g	YAb/glun mg/g
Batch	13.25	72.31	0.84	77.73	4.77	63.17	344.9
Fed-batch A	6.48	80.42	0.42	115.24	5.29	34.26	425.57
Fed-batch B	8.53	104.54	0.38	154.32	5.16	43.97	539.13
Perfusion	13.87	291.94	0.30	258.93	4.69	44.72	721.40

Y_Vx/Gluc yield coefficient of rCHO-C12 cells to glucose consumption, Y_Vx/Gln yield coefficient of rCHO-C12 cells to glutamine consumption, Y_Lac/Gluc yield coefficient of lactate production to glucose consumption, Y_Amm/Gln yield coefficient of ammonia production to glutamine consumption, Y_Ab/Vx yield coefficient of rCHO-C12 cells to antibody, Y_Ab/Gluc mean yield coefficient of antibody to consumed glucose, Y_Ab/Gln mean yield coefficient of antibody to consumed Gln