Fig. 2.

Flow cytometric analysis of haematopoietic stem cell surface antigen (CD34) in the UCB derived HSC was performed using the labelled antibody anti-CD34 or control IgG1 as indicated. The fluorescence of the conjugated monoclonal antibodies (anti CD34-PE and IgG1-PE) as well as side scatter signals were measured on dot plot. The ratios were calculated by the EPICS XL/MCS flowcytometer. The upper 6 panel represent the CD34⁺ cell population (1%) in the control sample (before MACS). The lower 6 panel represent the CD34⁺ cell population enriched after MACS was 4.5% on the average