Fig. 3.

Diagram of the EB growth of the 4 hESC lines when cultured in agarose 3-D matrix for 8 weeks. a Growth of the hESC lines SA002, AS034, SA167, SA121 when cultured in agarose for 8 weeks. All measure points represent the mean of six representative EBs. (filled circle) symbols the average size of the 4 hESC lines after 1 week of suspension culture from Fig. 2. (Open square) symbols the average size of the 4 hESC lines after 1 week of hanging droplet culture from Fig. 2. b The EBs from the 4 hESC lines; SA002, AS034, SA121 and SA167 showed all intact nuclei (black arrows) in the middle of the EBs cultured in agarose 3-D matrix for 8 weeks, here represented by the AS0034 hESC line. Staining = Hematoxylin-Eosin. Bar 10 μm