Fig. 2.

Effect of DMSO on spontaneous oocyte aging. a The rate of fragmentation increased in a time-dependent manner in oocytes cultured in M16 control medium. Spontaneous oocyte fragmentation was inhibited in a concentration-dependent manner by the addition of DMSO to the M16 medium. Data represent the mean ± SEM (n = 3), and values with different superscripts are significantly different (P < 0.05). b The frequencies of four different oocyte phenotypes were evaluated after culture for 3 days in the presence or absence of DMSO. DMSO increased the frequency of non-activated oocytes, including those with condensed chromosomes and meiotic spindles, in a concentration-dependent manner