Fig. 3.

Fluorescence photomicrographs of oocytes stained with anti-tubulin antibody and DAPI. Ovulated oocytes were fixed within 2 h of ovulation. Most single-cell oocytes cultured for 3 days in vitro were activated and contained multiple nuclei. Most oocytes cultured with DMSO for 3 days contained abnormal spindles and condensed chromosomes. Scale bar: 50 μm