Table 1.
Determined IC50 values for inhibition of human sEH for a small library of 23 different urea derivatives. The results from the developed LC–MS assay are compared with those from the commonly used fluorescence (FD) assay
| Inhibitor no. | IC50 FD assay (nmol L−1) | IC50 LC–MS assay (nmol L−1) |
|---|---|---|
| 1 | ≤1 | 2.4 ± 0.6 |
| 2 | ≤1 | 0.7 ± 0.3 |
| 3 | ≤1 | 5.4 ± 0.9 |
| 4 | ≤1 | 1.8 ± 0.5 |
| 5 | ≤1 | 4.9 ± 1.3 |
| 6 | ≤1 | 1.3 ± 0.4 |
| 7 | ≤1 | 1.3 ± 0.3 |
| 8 | ≤1 | 1.4 ± 0.1 |
| 9 | ≤1 | 5.0 ± 0.1 |
| 10 | ≤1 | 2.2 ± 0.3 |
| 11 | 2 | 3.2 ± 0.3 |
| 12 | 4 | 4.4 ± 0.2 |
| 13 | 3 | 7.3 ± 3.1 |
| 14 | 3 | 22.5 ± 3.8 |
| 15 | 14 | 13.1 ± 5.4 |
| 16 | 15 | 16 ± 0.4 |
| 17 | 28 | 18.2 ± 2.8 |
| 18 | 39 | 24 ± 5 |
| 19 | 133 | 171 ± 25 |
| 20 | 284 | 368 ± 51 |
| 21 | 684 | 111 ± 20 |
| 22 | 1011 | 567 ± 81 |
| 23 | 1441 | 1323 ± 26 |