Figure 5.

Insulin-Stimulated Glucose Transport in Ex Vivo Muscles and Primary Adipocytes

(A) Insulin-stimulated glucose transport in ex vivo soleus muscles. The soleus muscles were isolated from male mice (10–12 weeks old) stimulated with or without 0.1 mU/ml of insulin and used for glucose transport assay. A fold increase of the rates of glucose transport and an increase of the rates of glucose transport upon insulin stimulation over the basal state were deduced from the glucose transport assay. The values are given as the mean ± SEM (n = 7). Asterisk indicates p < 0.05. a indicates p < 0.05 versus WT (insulin).

(B) Insulin-stimulated glucose transport in the ex vivo EDL muscles. The EDL muscles were isolated from both wild-type and AS160 knockin mice (8–9 weeks old) stimulated with or without 0.1 mU/ml of insulin and used for glucose transport assay. A fold increase of the rates of glucose transport and an increase of the rates of glucose transport upon insulin stimulation over the basal state were deduced from the glucose transport assay. Asterisk indicates p < 0.05.

(C) Insulin-stimulated glucose transport in primary adipocytes. The primary adipocytes were isolated from the epididymal fat pads of male mice (10 weeks old) stimulated with or without 16.7 mU/ml insulin for 30 min and used for glucose transport assay. The values are given as the mean ± SEM (n = 8). Asterisk indicates indicates p < 0.05. a indicates p < 0.05 versus WT (insulin).

(D) In vivo muscle glucose uptake. Male mice at 12 to 14 weeks of age were subject to intraperitoneal injection of glucose (2 mg/g body weight) containing 2-deoxy-[³H]-glucose. At 60 min after injection, both tibialis anterior and quadriceps muscles were removed and used to determine muscle glucose uptake. The values are given as the mean ± SEM (n = 5). ^∗ indicates p < 0.05 (t test).