Fig. 1.

Impact of cryopreservation on cervical cytobrush-derived CD3⁺ T cell viability measured by Annexin V and propidium iodide staining. (A) Representative figure of cervical cytobrush CD3⁺ T cells stained with propidium iodide and Annexin V to differentiate live (Annexin−PI−), early apoptotic (Annexin + PI−), late apoptotic (Annexin + PI+) or dead (Annexin−PI+) cells. (B) Stacked bar graph showing the proportion of CD3⁺ cells that were live, apoptotic or dead in cervical cytobrush samples from HIV-infected and uninfected women that were (i) processed and stained immediately (HIV + only), (ii) cryo-preserved then thawed, or (iii) thawed and rested.