Fig. 3.

HOCl–HDL-induced Egr-1 DNA binding activity in endothelial cells. EMSA of HOCl–HDL-induced Egr-1 DNA binding activity. Cells were stimulated with 100 μg/ml HOCl–HDL for the indicated times, nuclear extracts were isolated and incubated with radioactively labelled oligonucleotides of the Egr-1 consensus-binding site ([³²P]-oligo-Egr-1). For competition experiments extracts were preincubated with a 20-fold molar excess of unlabeled oligonucleotides (oligo Egr-1) specific for Egr-1. Lanes 1 and 2 represents non-stimulated controls, i.e. cells in the absence of lipoproteins. One experiment out of three is shown.