Fig. 4.

HOCl–HDL-induced Egr-1 nuclear translocation in endothelial cells. (A and B) Cells were incubated with 100 μg/ml lipoprotein for the indicated times. Cells were lysed, nuclear and cytosolic extracts were isolated and equal amounts of proteins were subjected to Western blot analysis for Egr-1 and Nrf2. After stripping membranes, β-actin or lamin was used as cytosolic or nuclear loading control. (A) Lanes 1 and 8 represents non-stimulated controls. (B) Lane 1 represents non-stimulated controls. (A and B) One experiment out of three is shown.