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. 2011 May 1;509(1-4):16–25. doi: 10.1016/j.abb.2011.02.016

Fig. 6.

Fig. 6

Real-time RT-PCR and Western blot analysis of lipoprotein-induced HO-1 expression in endothelial cells. (A) Cells were stimulated with 100 μg/ml lipoprotein for the indicated times. Equal amounts of RNA were subjected to Real-time RT-PCR. HO-1 expression was normalized to GAPDH (∗∗∗p < 0.001). (B) Cells were incubated with 100 μg/ml lipoprotein for the indicated times. (C) Cells were incubated with indicated concentrations of HOCl–HDL for 12 h. (D) Cells were incubated with 100 μg/ml lipoprotein for 12 h. (E) Cells were preincubated for 30 min with 25 μM PD98059 or 10 μM SB203580 prior to stimulation with 100 μg/ml HOCl–HDL for 12 h. Lane 4 represents non-stimulated controls, i.e. cells in the absence of lipoproteins. (B–D) Lane 1 represents non-stimulated controls. (B–E) Cells were lysed and equal amounts of proteins were subjected to Western blot analysis for HO-1. After stripping membranes, β-actin was used as a loading control. (A–E) One representative experiment (performed in triplicate A) out of three is shown.