Skip to main content
. 2011 Feb;92(Pt 2):421–431. doi: 10.1099/vir.0.025494-0

Fig. 6.

Fig. 6.

Overexpression of XBP-1s in PEL and BL cell lines does not increase the mRNA expression levels of BZLF1 and BRLF1. Q-RT-PCR was used to determine the expression of BZLF1 mRNA (open bars), BRLF1 mRNA (grey bars) and K-RTA mRNA (black bars) after transduction with XBP-1s lentivirus or treatment with chemical inducers for (a) Akata and (b) JSC-1 cell lines. (a) Neither XBP-1s overexpression nor TPA, VPA or DTT treatments were able to increase BZLF1 or BRLF1 mRNA expression. BCR cross-linking induced BZLF mRNA expression (P<0.05, two-tailed t-test). (b) In JSC-1 cells, expression of XBP-1s only increased K-RTA mRNA expression. TPA, VPA and DTT treatment also increased K-RTA mRNA expression significantly (P<0.05, two tailed t-test). TPA-treated JSC-1 cells expressed BZLF1 mRNA compared with negative control (P<0.05, two tailed t-test).