Fig. 2.

Localization of syntaxin 6 to the inclusions of multiple chlamydial species. C2BBe1 cells were seeded onto coverslips and transfected with mCherry-syntaxin 6 or with eGFP-syntaxin 6, then infected with C. trachomatis serovar L2 (m.o.i. 6 : 1), C. muridarum (m.o.i. 0.1 : 1), C. caviae (m.o.i. 0.2 : 1), C. pneumoniae (m.o.i. 13 : 1) or Coxiella burnetii Nile Mile phase II (m.o.i. 50 : 1) as described in Methods. To terminate the infections, cells were fixed in methanol and processed for indirect immunofluorescence to detect the organisms (green). Samples were visualized with an LSM 510 Laser Module Zeiss Axiovert 200M confocal microscope. Bar, 10 μm.