Fig. 3.

Chlamydial protein synthesis requirement for syntaxin 6 localization to the inclusion. C2BBe1 cells were seeded onto glass coverslips in 24-well plates 48 h prior to infection with C. trachomatis serovar L2 (m.o.i. 9 : 1). After 18 h, cells were either fixed in absolute ethanol (−Chlor) or treated with 200 μg ml⁻¹ for an additional 24 h (+Chlor), then fixed in absolute ethanol and processed for indirect immunofluorescence essentially as described in Methods. IncG staining was used to identify the inclusion. Samples were visualized with an LSM 510 Laser Module Zeiss Axiovert 200M confocal microscope. Bar, 10 μm.