Fig. 2.

Transcription of the ccm, petC, cyc4 and cycB genes. (a) Expression of ccm transcripts. Wild-type 130b was grown in deferrated CDM (CDM–Fe) or standard BYE broth (BYE), and then RNA was analysed by RT-PCR utilizing primers specific to ccmC, ccmE, ccmG or ccmH. That the PCR products obtained resulted from mRNA templates was confirmed by the lack of product obtained when the reaction did not incorporate RT (–RT lanes). In additional experiments, ccmB and ccmF transcripts were detected in 130b growing in CDM–Fe (data not shown). (b) Expression of the c-type cytochrome genes. RNA from the wild-type was analysed by RT-PCR utilizing primers specific to petC, cyc4 or cycB. The results presented are representative of three independent experiments.