Fig. 5.

Growth of L. pneumophila wild-type and c-type cytochrome mutants on BCYE agar. We spotted 10 μl aliquots from 10-fold serial dilutions of wild-type 130b, ccmC mutant NU295, petC mutant NU375, cyc4 mutant NU379, cycB mutant NU381 and lbtA mutant NU302 onto standard BCYE agar (a) or BCYE lacking its usual iron supplement (b). After 4 days, growth was recorded. Each strain (i.e. each column of growth depicted here) was spotted on its own plate, to prevent diffusible factors produced by some strains from stimulating the growth of others nearby and thereby confounding mutant analysis. The results shown are representative of three experiments.