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The effects of the ampR mutation on algT/U transcription. The promoter fusion PalgT/U-lacZ was introduced into Alg− PAO1, Alg− PAOampR, Alg+ PDO300 and Alg+ PDOampR. Induction was carried out using 500 μg benzylpenicillin ml−1 and the β-galactosidase activity was determined in Miller units after 30 min incubation. The basal level of expression was detected in the promoterless lacZ vector, pLP170.
