Fig. 4.

Kinetics of the paralysis of C. elegans by P. aeruginosa Alg⁻ PAO1 wild-type strain (—), Alg⁻ PAOampR (– ^{. .} –), complemented Alg⁻ PAOampR(pAmpR) (– ^. –), Alg⁺ PDO300 (-^.-), Alg⁺ PDOampR (--) and complemented Alg⁺ PDOampR(pAmpR) (^…). L4 stage larval hermaphrodite Bristol N2 C. elegans were placed on each brain heart infusion agar plate containing a bacterial lawn and scored for dead worms by microscopic examination. E. coli OP50 (— —) was used as a negative control. Values are the mean±sd of triplicate analyses. Results were statistically significant (P<0.05 for PAO1 vs PAOΔampR at 2 h, and PDO300 and PDOampR at 4 h).