. 2011 Feb;60(Pt 2):147–156. doi: 10.1099/jmm.0.021600-0

Table 1.

Bacterial strains, plasmids and primers used in this study

Strain/plasmid	Genotype	Reference
*E. coli*
DH5α	F⁻φ80dlacZΔM15 Δ(lacZYA–argF) U169 deoR recA1 endA1 hsdR17(r_k⁻, m_k⁺) phoA supE44 λ- thi-1 gyrA96 relA1	New England Biolabs
TOP10F′	F′[lacI^q, Tn10(Tet^R)] mcrA Δ(mrr-hsdRMS-mcrBC)φ80dlacZΔM15 ΔlacX74 deoR recA1 araD139 D(ara-leu)7697 galU galK rpsL (Str^R) endA1 nupG	Invitrogen
*P. aeruginosa*
PAO1	Prototype; Alg⁻	Holloway & Morgan (1986)
PDO300	PAOmucA22; Alg⁺	Mathee et al. (1999)
PKM805	PAOmucA22algT24-1; Alg⁻	PDOalgT; PDO300 derivative; Ramos et al. (2003)
PKM300	PAOampR : : aacCI; Alg⁻	PAOampR; Kong et al. (2005b)
PKM307	PAOmucA22 ampR : : aacCI; Alg⁺	PDOampR; this study
PKM308	PAOmucA22 attB : : P_ampC–lacZ; Tc^R, Alg⁺	PDO300 derivative; this study
PKM309	PAOmucA22 attB : : P_ampR–lacZ; Tc^R, Alg⁺	PDO300 derivative; this study
PKM310	PAOmucA22 ampR : : aacCI attB : : P_ampC-lacZ; Gm^R, Tc^R, Alg⁺	PDOampR derivative; this study
PKM311	PAOmucA22 ampR : : aacCI attB : : P_ampR–lacZ; Gm^R, Tc^R, Alg⁺	PDOampR derivative; this study

Plasmids
Mini-CTX-lacZ	Tc^R; integration-proficient vector for single-copy chromosomal lacZ fusion	Becher & Schweizer (2000)
pEX100T	Ap^R; sacB oriT	Schweizer & Hoang (1995)
pGEMEX-1	Ap^R; ColE1ori lacZα	Promega
pKMG37	Ap^R; pQF50 containing P_algT-lacZ transcriptional fusion	Mathee et al. (1997)
pLP170	Ap^R; lacZ transcriptional fusion vector that contains an RNase III splice sequence positioned between the MCS and lacZ	Preston et al. (1997)
pLPLA	Ap^R; pLP170 containing P_lasA-lacZ transcriptional fusion	Preston et al. (1997)
pLPLB	Ap^R; pLP170 containing P_lasB-lacZ transcriptional fusion	Preston et al. (1997)
pLPR1	Ap^R; pLP170 containing P_rhlI-lacZ transcriptional fusion	Van Delden & Iglewski (1998)
pME6030	Tc^R; oriV_pVS1oriV_p15AoriT	Heeb et al. (2000)
pPCS223	Ap^R; pLP170 containing P_lasI-lacZ transcriptional fusion	Van Delden & Iglewski (1998)
pPCS1001	Ap^R; pLP170 containing P_lasR-lacZ transcriptional fusion	Pesci et al. (1997)
pPCS1002	Ap^R; pLP170 containing P_rhlR-lacZ transcriptional fusion	Pesci et al. (1997)
pQF50	Ap^R; broad-host-range vector with promoterless lacZ	Farinha & Kropinski (1990)
pRK2013	Km^R; ColE1ori-Tra (RK2)⁺	Figurski & Helinski (1979)
pSJ01	Ap^R; pGEMEX-1 with a 1220 bp EcoRI–BamHI flanked fragment containing ampR	Kong et al. (2005b)
pSJ06	Ap^R; pME6030 with a 1220 bp EcoRI–BamHI flanked fragment containing ampR (referred to as pAmpR)	Kong et al. (2005b)
pSJ07	Ap^R; pEX100T derivative with ampR : : aacCI	Kong et al. (2005b)
pSJ09	Ap^R, Gm^R; pGEMEX-1 with a 330 bp EcoRI–BamHI flanked fragment containing ampC-ampR intergenic region	Kong et al. (2005b)
pSJ10	Tc^R; CTX-lacZ fused with ampC promoter, P_ampC	Kong et al. (2005b)
pSJ11	Tc^R; CTX-lacZ fused with ampR promoter, P_ampR	Kong et al. (2005b)
pUCGm	Ap^R, Gm^R; pUC19 derivative containing gentamicin cassette	Schweizer (1993)

Primers
SBJ01ampRFor*	5′-GGAATTCTGGCGAACAGCAGTGTGGAAGCGG-3′
SBJ02ampRRev*	5′-CGGGATCCATTCCAATCACAACCCCAACGCC-3′
SBJ03ampCRFor*	5′-GGAATTCTGAGGCCGCGCGGCAGACGCTTGAACA-3′
SBJ04ampCRRev*	5′-CGGGATCCCATGAGGATTGGCGTCCTTTG-3′
DBS_QRTAmpRF	5′-CATTGGCCTTCATCACCGGTTGTA-3′
DBS_QRTAmpRR	5′-GGTTTCTCATGCAGCCCACGACAA-3′

*The italicized portion of the sequence indicates a restriction site in a PCR product prepared with the primer.