Table 3.
Inducer* | Pyocyanin [μg (μg total protein)−1]† | LasA [ΔOD600 h−1 (μg protein)−1]‡ | PlasA-lacZ (Miller units)§ | ||
---|---|---|---|---|---|
− | + | − | + | − | |
Alg− PAO1|| | 0.285±0.219 | 2.293±0.216¶ | 0.310±0.065 | 0.317±0.059 | 790.0±128.9 |
Alg− PAOampR|| | 2.934±0.761# | 3.317±0.638 | 1.109±0.099# | 0.951±0.045# | 1970.5±312.6# |
Alg+ PDO300 | 0.102±0.017** | 0.324±0.051 | 0.135±0.011 | 0.147±0.024 | 246.5±26.5 |
Alg+ PDOampR | 0.538±0.026†† | 2.518±0.640†† | 0.268±0.017 | 0.143±0.025 | 536.0±19.0 |
*Induction was carried out using 500 μg benzylpenicillin ml−1 for P. aeruginosa.
†Pyocyanin concentrations were expressed as μg pyocyanin produced (μg total protein)−1.
‡LasA activities were determined as the reduction of OD600 over a period of 1 h (μg total protein)−1.
§β-Galactosidase assays were performed in a high-throughput 96-well array and the results expressed in Miller units.
||These data are presented in a previous paper (Kong et al., 2005b); they are included here for comparison.
¶P<0.05 between non-induced and induced in the same strain.
#P<0.05 between Alg− PAO1 and Alg− PAOampR under the same conditions.
**P<0.05 between Alg− PAO1 and Alg+ PDO300 under the same conditions.
††P<0.05 between Alg+ PDO300 and Alg+ PDOampR under the same conditions.