Figure 1.

Characterization of OPCs and astrocytes. A–C, All purified OPCs express hPAP (A) and OPC markers O4 (A, B), olig2 (B), A2B5 (C), and Sox10 (C). D, E, OPCs differentiate into O1⁺ OLs after withdrawal of FGF2 and PDGF-aa for 3 d (D), and MBP⁺ OLs to form myelin around the axons after coculture with DRG neurons for 2 weeks (E). F, All purified astrocytes from 1 month postinjury spinal cord express GFAP. Some astrocytes are still proliferating as evidenced by BrdU incorporation. Scale bar, 50 μm.