Figure 8.

Representative Western blots of Mdr1 (A,B), Mrp1 (C,D) and Mrp4 (E,F) in wild-type (WT) and mtDNA-depleted (Rho) Hepa 1–6 mouse hepatoma cells incubated with the indicated concentrations of glycoursodeoxycholic acid (GUDCA) (A,C,E) or glycochenodeoxycholic acid (GCDCA) (B,D,F) for 48 h. These results were confirmed in two additional experiments. For Mrp1 and Mrp4, total cell lysates were used and Western blots were normalized using Gapdh detection. Owing to the low signal found for Mdr1, the Western blots for this protein were carried out with crude membrane, and Na⁺/K⁺-ATPase detection was used to normalize the results.