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. 2011 Apr;162(8):1832–1842. doi: 10.1111/j.1476-5381.2011.01204.x

Figure 3.

Figure 3

Effects of HS and HSF-1 overexpression on SAP97 mRNA expression. HL-1 cells were treated with HS (at 42°C for 1 h) (left), HSF-1 Wt and mutant R71G overexpression (right), then real-time PCR analysis were performed with SAP97 and β-actin primers. The mean ratio (±SEM) of each mRNA quantity normalized to β-actin (n = 6 for control and each treatment groups) is shown. Statistical significance of the differences among each group was tested by Student's t-test: *P < 0.05, **P < 0.01. PCR, polymerase chain reaction.