Figure 5.
siRNA-SAP97 regulation in HSF-1-induced increases in expression of HSF-1, SAP97 and Kv1.5 proteins. HL-1 cells transfected with siRNA against SAP97 or scrambled siRNA were treated with Wt-HSF-1 plasmid and analyzed by Western blotting using anti-HSF-1, SAP97 and Kv1.5 antibodies. The blots at the bottom were reprobed with anti-β-actin as internal control. The mean ratio (±SEM) of each protein level normalized to β-actin (n = 3 for control and each treatment groups) is shown (right). Statistical significance of the differences among each group was tested by Student's t-test: *P < 0.05; N.S., not statistically significant; siRNA, small interfering RNA.