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. 2011 May;178(5):2344–2356. doi: 10.1016/j.ajpath.2011.01.020

Figure 6.

Figure 6

STAT5 S710F promotes expression of OSM. A: Ton.Stat5.S710F cells were incubated in the absence [control (Co)] or presence (Doxy) of doxycycline (1 μg/mL) for 24 hours. Cells were then harvested, and expression of total STAT5 and phosphorylated STAT5 (pSTAT5) was determined. Actin served as a loading control. B: Ton.Stat5.S710F cells kept without IL-3 were cultured in the absence (squares) or presence (circles) of doxycycline (without IL-3), and the number of viable cells was determined every 24 hours. C: Ton.Stat5.S710F cells were cultured in the presence or absence of doxycycline with or without IL-3 for 24 hours. Expression of OSM was then determined by real-time PCR. Results represent the mean ± SD of three independent experiments. D:KIT D816V+ HMC-1 cells were lentivirally transduced with STAT5 S710F. At 24 hours after transduction, cells were selected with puromycin (2 μg/mL) for 48 hours. Expression of OSM was then determined by quantitative real-time PCR. Results represent the mean ± SD of three independent experiments. *P < 0.05.