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. 2011 May;178(5):2344–2356. doi: 10.1016/j.ajpath.2011.01.020

Figure 7.

Figure 7

Effects of wild-type KIT on activation of STAT5 and expression of OSM. (A, B) CD34+ progenitor cells isolated from cord blood (n = 5) were cultured in the presence of SCF and IL-6 (both 100 ng/mL). Box plots show expression of OSM (A) or histidine decarboxylase (HDC; B) mRNA levels as determined by real-time PCR on day 1, 10, and 40. *P < 0.05. C: Primary human lung mast cells cultured in the presence of SCF were spun onto cytospin slides and expression of phosphorylated STAT5 was analyzed by immunocytochemistry using the anti-pSTAT5a/b antibody AX1. KIT D816V+ HMC-1 cells were included as a positive control. D: Primary human lung mast cells and KIT D816V+ HMC-1 cells were analyzed for expression of the OSM protein by immunocytochemistry as described above. E and F: Primary lung mast cells obtained from four different patients and KIT D816V+ HMC-1 cells (five independent experiments) were analyzed for expression of PIM1 (E) or BCL-XL (F) mRNA levels by real-time PCR. Results are shown as box plots. *P < 0.05.