Figure 8.

OSM derived from KIT D816V⁺ cells stimulates proliferation of mesenchymal cells. A–C: Human microvascular endothelial cells (HMEC-1 cells; A), human immortalized osteoblasts (B) and primary human bone marrow fibroblasts (C) were incubated with supernatant (sup) from KIT D816V⁻ HMC-1 or KIT D816V⁺ HMC-1 cells. In addition, cells were treated with supernatant from KIT D816V⁺ HMC-1 cells preincubated with a neutralizing antibody against OSM (nAb) at a concentration of 10 μg/mL. Cells were incubated with supernatants for 72 hours. Proliferation of cells was then determined by [³H]thymidine incorporation assay. Results represent the mean ± SD of triplicates and represent one of three almost identical experiments. *P < 0.05.