Figure 3.

ChIP assay of dnPPARγ-Flag fusion protein on the promoters of Api6, IL-1β, IL-6, MMP12 and TNF-α inflammatory molecule genes in CCSP-rtTA/(tetO)₇-CMV-dnPPARγ bitransgenic mice. A: The amplified regions that contain putative PPARγ DNA binding sites (PPAR E) within the promoters of genes encoding Api6, IL-1β, IL-6, MMP12, and TNF-α. B: Statistical real-time PCR analyses of ChIP assay from dnPPARγ-Flag fusion protein and promoters of Api6, IL-1β, IL-6, MMP12, and TNF-α (mice, n = 4 to 6). Relative binding levels were determined by 2^(−ΔΔCt), in which ΔΔC_t = ΔC_t(+DOX) − ΔC_t(−DOX). The testing sample C_t value was normalized by each input DNA fraction C_t value, as ΔC_t = C_t(testing) − (C_t(input) − log_{2(input dilution factor)}). Data are reported as means ± SD. *P < 0.05; **P < 0.01. −DOX, doxycycline-untreated bitransgenic mice; +DOX, doxycycline-treated bitransgenic mice.