Table 2.

Phosphorylated Activation of Intracellular Signaling Molecules in Lung Alveolar Type II Epithelial Cells from CCSP-rtTA/(tetO)₇-CMV-dnPPARγ Bitransgenic Mice

	Percentage ratio (%)		MFI ratio (%)
	−DOX	+DOX	−DOX	+DOX
pStat1	10.37 ± 3.18	47.32 ± 5.92⁎⁎	22.42 ± 9.18	66.65 ± 16.11⁎⁎
pStat2	9.54 ± 4.23	33.47 ± 6.12⁎⁎	12.62 ± 2.79	27.78 ± 5.45⁎⁎
pStat3	6.47 ± 2.1	39.01 ± 8.31⁎⁎	11.22 ± 3.13	41.22 ± 7.22⁎⁎
pStat4	11.18 ± 2.88	42.11 ± 4.35⁎⁎	11.90 ± 2.74	14.72 ± 3.85
pStat5	23.79 ± 7.15	9.50 ± 4.69⁎	30.09 ± 10.65	13.48 ± 3.01⁎
pStat6	25.81 ± 6.8	10.07 ± 4.77⁎	20.20 ± 5.22	18.48 ± 4.52
pErk	10.87 ± 1.55	33.78 ± 3.33⁎⁎	18.86 ± 3.96	51.30 ± 7.76⁎⁎
pP38	14.51 ± 3.56	41.11 ± 10.49⁎⁎	24.79 ± 5.56	59.05 ± 9.55⁎⁎

−DOX, doxycycline-untreated bitransgenic mice; +DOX, doxycycline-treated bitransgenic mice.

Cells isolated from the lung were stained with anti-SP-C antibody, followed by intranuclear staining with anti-phospho-Stat1-6, anti-phospho-Erk, or anti-phospho-P38 antibody, and Stat1-6, Erk, or P38 antibody as described under Materials and Methods. Numbers of percentage ratio and mean fluorescent intensity (MFI) ratio between phospho-proteins and total proteins in gated SP-C positive cells from four independent experiments (n = 4) were calculated and statistically analyzed.

^⁎P < 0.05;

^⁎⁎P < 0.01.