Figure 4.

Visualization of retinal blood vessels leakage after intravascular perfusion with Evans blue dye. In control retinas (A, detail in D), the superficial (in focus) and deeper vasculature was sharply outlined by the dye, showing very low levels of background fluorescence. At 6 weeks of diabetes, in retinas from eyes submitted to a sham procedure (B, detail in E), focal sites of leakage were noted, with the dye diffusely distributed through the retinal parenchyma. The severity of the vascular permeability was markedly reduced in diabetic eyes submitted to ischemia pulses (C, detail in F), with minor focal leakage. Scale bar: 500 μm (A–C); 50 μm (D–F). Shown are photographs representative of four eyes per group.