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. 2011 May;178(5):2236–2251. doi: 10.1016/j.ajpath.2011.01.038

Figure 6.

Figure 6

GATA-6 immunoprecipitates with the GATA-6 consensus motif in the caveolin-1 (CAV1) promoter at ChIP analysis. A: Chromatin samples were prepared from control, sham, and PBOO mouse bladders, followed by immunoprecipitation with anti-GATA-6 or preimmune serum. Precipitated fragments were PCR-amplified using primers specific for GATA-6 binding motif on mouse CAV1 promoter (upper panel) or CAV1 exon-1 (lower panel) as negative control. B: Intensities of the bands (shown in A) are presented as percentage of input chromatin (average of six healthy, six sham, and six PBOO samples). C: Chromatin samples were prepared from healthy and BPH human bladder samples as in A. Precipitated fragments were PCR-amplified using primers specific for GATA-6 binding motif on human CAV1 promoter. D: Intensity of the bands (C) is presented as percentage of input chromatin (six healthy men and six men with BPH-induced PBOO, shown individually). I, input; PI, precipitation with preimmune serum.