Figure 3.

SPLUNC1 inhibits Mp growth and P1 adhesin mRNA. A: Recombinant hSPLUNC1 protein, 10 μg/mL, significantly reduces Mp (10⁶ CFUs/mL) growth when co-incubated in 96-well plates for 24 hours. B: Mp (10⁶ CFUs/mL) was incubated with BSA, 10 μg/mL, or recombinant hSPLUNC1, 10 μg/mL, for 24 hours at 35°C. Bacterial RNA was extracted, and quantitative real-time PCR was performed for P1 adhesin mRNA. 16S was used as a housekeeping gene to normalize P1 adhesin expression. A and B, Data represent results obtained from three independent experiments. CFUs, colony-forming units. **P < 0.01.