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. 2011 May;178(5):2159–2167. doi: 10.1016/j.ajpath.2011.01.026

Figure 5.

Figure 5

Effect of hSPLUNC1 overexpression on lung inflammation after Mp infection. A: hSPLUNC1 overexpression in mouse lungs decreases the severity of lung inflammation elicited by Mp. Paraffin sections 4-μm thick were stained with H&E, analyzed using light microscopy, and assigned a histopathology score in a blinded fashion. The groups consisted of saline solution–treated (Sal) or Mp-infected (Mp) hSPLUNC1 transgene-positive (Tg+) or transgene-negative (Tg−) mice (n = 10 mice per group). B: Representative histopathologic microphotographs of lung tissues (original magnification ×200; scale bars = 100 μm). Upper left panel: Saline solution–treated transgene-positive mice demonstrated no inflammation. Bottom left panel: Mp-infected transgene-positive mice exhibited moderate peribronchial leukocyte infiltration (arrow). Upper right panel: Saline solution–treated transgene-negative mice exhibited no inflammation. Bottom right panel: Mp-infected transgene-negative mice exhibited significant peribronchial and airway lumen leukocyte accumulation and severe pneumonia (arrows). C: Neutrophils in BAL fluid from saline solution–treated or Mp-infected transgene-positive or transgene-negative mice (n = 10 mice per group). D: Neutrophil elastase activity levels normalized to neutrophil numbers in BAL fluid from saline solution–treated or Mp-infected transgene-positive or transgene-negative mice (n = 10 mice per group). ND, not detected. *P < 0.05, †P < 0.005, ††P < 0.0001.