Figure 4.

Protective effect of NACA against ROS production and lipid peroxidation in ARPE-19 cells. A: Cells were pretreated with media or NACA for 24 hours followed by exposure to 0.4 mmol/L tBHP. Fluorescence measured is directly correlated to ROS present in the cell. B: MDA levels in tBHP-exposed cells. Cells were pretreated with 5 mmol/L NACA for 24 hours, followed by exposure to tBHP. The control media group was incubated in media for 28 hours, The NACA group was exposed to NACA for 24 hours, followed by media alone for 4 hours. Cells were collected, and MDA levels, a biomarker that measures the level of oxidative stress in a cell due to lipid peroxidation, were measured. *P < 0.01. Error bars represent SE.