AIC effects on diabetic adipose tissue with chronic hypoxia, part I. A: Time course of adipose tissue pO2 in db/db mice (n = 5). B: Whole-mount histologic analysis of living adipose tissue. Harvested, unfixed tissue samples were stained with BODIPY (adipocytes; green), lectin (ECs; red), and Hoechst 33342 (nuclei; blue). Scale bars = 50 μm. C: Histologic sections were stained with lectin (ECs; red) and Hoechst 33342 (nuclei; blue), and capillary density was calculated (n = 6). *P < 0.05; **P < 0.01. Scale bars = 100 μm. Error bars represent SEM.