RSF1 gene is overexpressed in oral squamous cell carcinoma (OSCC). A: Representative examples of OSCC were stained with H&E. Based on IHC, RSF-1 immunoreactivity on tumor tissues was scored as 0, +1, +2, +3, or + 4. B: Quantitative real-time PCR (qPCR) analysis indicated higher expression levels of the RSF1 gene in OSCC than in normal epithelial cells. C:RSF1 amplification was frequently found in OSCC tissues analyzed by qPCR. Arrowhead indicates the OSCC sample with highest RSF1 content and gene expression level.