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. 2011 May;178(5):2407–2415. doi: 10.1016/j.ajpath.2011.01.043

Figure 5.

Figure 5

Assessment of RSF1 gene knockdown in RSF-1 expressing oral squamous cell carcinoma (OSCC) cells. A: Western blot analysis revealed the highest expression level of RSF-1 in SAT cells, a moderate level in KON cells, and undetectable levels in SSC4 and CAL27 cells. B: Western blot analysis shows an efficient reduction of RSF-1 protein by RSF-1 specific short-interfering RNA (siRNA). Cells treated with buffer alone (mock) or scramble siRNA were used as the controls. C: Differential growth suppression was found when cells were treated with RSF-1 specific siRNA (blank circles). Cells treated with scramble siRNA were used as the controls (filled circles). DNA synthesis was monitored by (D) Bromodeoxyuridine (BrdU) incorporation and (E) cell death induction was monitored by annexin V staining 4 days after RSF-1-specific siRNA treatment. Cells treated with buffer alone (mock) or scramble siRNA were used as controls. F: Soft agar assay indicates that RSF-1 knockdown leaded to reduction of colony formation in SAT and KON cells, but caused very minor effects on CAL27 cells. Cells treated with scramble served as controls.