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. 2011 Apr 12;52(5):2343–2351. doi: 10.1167/iovs.10-6141

Figure 6.

Figure 6.

Restoration of VLGR1 expression in whirlin−/− retinas injected with AAV-whirlin. (A) A representative Western blot showing VLGR1 expression in the wild-type retina (whirlin+/+) and the whirlin−/− retina with different treatments: con inj, control subretinal injection with AAV-zsGreen, 10 weeks post injection; no inj, no injection; whirlin inj, subretinal injection with AAV-whirlin, 10 weeks post injection. The Vlgr1 null retina (Vlgr1−/−) was used as a negative control. The dystrophin signal on the same blot (two bands) was used as a sample loading control. (B) Quantification of VLGR1 signal intensities on the Western blot from wild-type retinas, whirlin−/− retinas with no subretinal injection (no inj), and whirlin−/− retinas at six months post injection of AAV-whirlin (whirlin inj). The difference between wild-type retinas and whirlin−/− retinas and the difference between whirlin−/− retinas with and without the subretinal injection of AAV-whirlin are statistically significant (marked with red asterisks, P < 0.05). The difference between wild-type retinas and whirlin−/− retinas injected with AAV-whirlin is not statistically significant (NS). The number of mice measured in each group is indicated at the bottom of the bars. The error bar represents the SEM. (C) Immunostaining of VLGR1 in the whirlin−/− retina at eight weeks post injection of AAV-whirlin (top panel, arrowheads), the wild-type retina (middle panel), and the whirlin−/− retina without any injection (bottom panel). The corresponding differential interference contrast images are shown on the left. OS, outer segment; IS, inner segment; ONL, outer nuclear layer. Scale bars, 10 μm. (D) Confocal images of VLGR1 double-stained with rootletin (left), acetylated α-tubulin (middle), and RPGR (right) in the wild-type retina (whirlin+/+, upper panels) and the whirlin−/− retina at six months post injection of AAV-whirlin (lower panels). These signal patterns strongly suggest that VLGR1 is correctly positioned at the PMC in the whirlin−/− retina injected with AAV-whirlin. Scale bars, 5 μm.