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. 2011 Apr 12;52(5):2379–2388. doi: 10.1167/iovs.10-6010

Figure 2.

Figure 2.

In situ hybridization for Ccl2 mRNA after exposure to BCL. (A) Expression of Ccl2 was not detectable in retinas from control, dim-light–reared animals. (B) Ccl2 mRNA expression was detected in isolated cells of the INL in the superior retina by 12 hours of BCL (arrowhead). (C) After exposure to 24 hours of BCL, Ccl2 mRNA was observed in cells with radially oriented processes throughout the INL (arrowheads). (D) Staining was not observed with the sense Ccl2 riboprobe in retinas after 24 hours of BCL. (E–G) The distribution of Ccl2 expression was detected in a superoinferior gradient, after BCL, with the most intense labeling detected in the superior retina. (H–J) Ccl2 expression (red, arrows) in sections counterimmunolabeled with anti-vimentin, showing double-labeled Müller cell processes (green; arrows, arrowheads) indicating that Ccl2 in the INL is expressed by Müller cells. INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; RPE, retinal pigment epithelium.