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Aged Er/+ mice showed evidence of meibomian gland atrophy. (A) Lipids in meibomian glands were stained with Sudan black (blue), and the slides were counterstained with H&E. Eye sections of WT and Er/+ mice at 1.0 and 4.5 months of age are shown. The images representative of serial sections through the glands. Arrows: sebaceous glands. (B) Immunostaining for 14-3-3σ (green) in a WT eye section containing sebaceous glands. The nuclei were counterstained with DAPI. Scale bar, 100 μm.
