Figure 1.

Representative PCR confirmation of the myelin PLP DM-20 variant transgene. The blood obtained by tail clipping of mice was subjected to PCR amplification. The DM-20 variant of the PLP transgene was confirmed by the presence of a band measuring approximately 400 bp. This band was absent in the control animals. The endogenous mouse β-globin gene was amplified and used as a control, giving an expected amplification product of 494 bp in both transgene and control animals.