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. 2010 Oct 1;183(5):649–658. doi: 10.1164/rccm.201005-0746OC

Figure 6.

Figure 6.

Early growth response (Egr)-1 regulates hypoxia-induced light chain-3B (LC3B) expression in vitro and in vivo, and protects against pulmonary hypertension during chronic hypoxia. (A) Pulmonary artery vascular smooth muscle cells (PASMC) were treated with hypoxia (1% O2) for 8 hours. Chromatin samples were immunoprecipitated with anti–Egr-1 and evaluated for factor binding to the LC3B promoter region. (B) Egr-1 expression relative to β-actin was evaluated in lung homogenate from normal patients (N) and patients with pulmonary hypertension (PH) by quantification of Western blots. Data represent mean ± SD (n = 5 normal, n = 10 PH). #P < 0.01. (C) The protein levels of Egr-1 in mouse lung tissues harvested from animals exposed to hypoxia and normoxia were evaluated by Western analysis. Data represent mean ± SD (n = 6). #P < 0.01. (D) Pulmonary artery endothelial cells (PAEC) were infected with Egr-1–specific siRNA or control siRNA, then treated with hypoxia (1% O2) for 0–24 hours. Expression of LC3B and Egr-1 was determined by immunoblotting with β-actin as the standard.