Table 3. The area and property of the interfaces in the Fab/antigen complexes.
Interface | bH1/VEGF | bH1/HER2 | Hercepin/HER2 | |||||||||
bH1 | VEGF | Combined | Fraction | bH1 | HER2 | Combined | Fraction | Herceptin | HER2 | Combined | Fraction | |
Polar | 311 | 295 | 606 | 40% | 308 | 282 | 591 | 37% | 307 | 308 | 614 | 40% |
Apolar | 438 | 462 | 900 | 60% | 470 | 518 | 988 | 63% | 441 | 469 | 910 | 60% |
Total | 749 | 757 | 1506 | 779 | 800 | 1579 | 747 | 777 | 1524 |
All numbers in Å2 unless otherwise indicated.
The polar and apolar area of the interfaces was assessed using XSAE (See Methods). The area was calculated using a probe with a radius of 1.4 Å.