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. 2011 Apr 22;6(4):e18809. doi: 10.1371/journal.pone.0018809

Figure 2. Biopanning of BCG specific γδ TCR recognized peptides from phage library using the transfected cells.

Figure 2

(A) The flow chart about screening and analysis of the peptides recognized by BCG specific γδ TCR. (B) Selection of BCG specific γδ TCR recognized positive phage clones by phage-ELISA. Representative data are shown in this figure. Dark arrow pointed bars represent the ratio of specific binding to nonspecific binding was more than 2, and corresponding phage clones were taken as positive clones. (C) Confirmation of BCG specific γδ TCR recognized positive clones by RT-PCR. The BCG specific γδ TCR recognized peptides were rescued by RT-PCR using phage specific primer provided in kit. 9 individual representative clones are shown in this figure. The RT-PCR products of positive clones were sequenced using specific sequencing primer.