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. 2011 Apr 22;6(4):e19003. doi: 10.1371/journal.pone.0019003

Figure 1. Negative stain electron microscopy of the CLC of F. tularensis.

Figure 1

Panels A and E: type B strain LVS_P10 grown at 32°C on Glc-CDMA; different cultures were examined on different days; Panel B: type A strain TI0902, passed and grown to enhance CLC expression as for LVS. In this and some other cases the CLC appeared to aggregate, which also occurred following isolation of the CLC; Panel C: glycosyl transferase mutant LVSΔ1423/1422_P10; Panel D: complemented strain LVSΔ1423/1422[1423/1422+]_P10; Panel F: LVS not passed in defined medium and grown on CDMA at 37°C; only a small amount of CLC is visible (arrow); Panel G: O-antigen mutant WbtIG191V_P17 grown as for LVS_P10; Panel H: O-antigen and CLC double mutant WbtIG191V_P17Δ1423/1422. Strains LVS_P10, WbtIG191V_P17, and type A strain TI0902_P10 have an electron dense layer surrounding their cells. This layer is missing in mutants LVSΔ1423/1422_P10 and WbtIG191V_P17Δ1423/1422, and is restored in complemented strain LVSΔ1423/1422[1423/1422+]_P10]. The bacteria were fixed in glutaraldehyde, and stained with uranyl acetate. Magnification is 20,000 X, and the scale bar is 500 nm.