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. 2011 Apr 22;6(4):e19003. doi: 10.1371/journal.pone.0019003

Figure 8. Intracellular survival of F. tularensis LVSΔ1423/1422 in J774A.1 cells.

Figure 8

The J774A.1 monolayer (approximately 4.5×105 macrophages/well) was infected with approximately 5.5×107 CFU/well of strain LVS (•), LVSΔ1423/1422 (▴), or LVSΔ1423/1422[1423/1422+] (X). Intracellular survival of the bacteria was determined at 0, 24, 48, and 72 h post-infection, as described in Materials and Methods. Data are shown on the log scale as the average number of bacteria recovered from dilutions of lysates of J774A.1 cells. The results shown were from two experiments tested in duplicate at each time point. The slopes for intracellular growth of the bacteria as log10 CFU/well between the 24th and the 48th hour for strains LVS, LVSΔ1423/1422, and LVSΔ1423/1422[1423/1422+] were +1.44, +0.73, and +0.97, respectively.