Table II.

DNA damage (%tail intensity) in lymphocytes of patients under anaesthesia with ISF collected at three time points and in vitro exposed to hydrogen peroxide

	Tail Intensity (%)
	T1	T2	T3
Lymphocytesa	2.6 (1.3–3.8)	2.6 (1.5–6.7)	2.4 (1.4–3.5)
H2O2b	10.3 (4.6–12.8)*	6.9 (2.7–7.8)*	5.5 (2.9–9.4)*

T₁: before anaesthesia; T₂: 2 h after the beginning of anaesthesia and T₃: on the first post-operative day. Data, obtained from 20 subjects, are expressed as median and quartiles.

^aWithout treatment with H₂O₂ (negative control).

^bH₂O₂ treatment: 100 μM (30 min at 4°C).

^*P < 0.05: H₂O₂ × lymphocytes.