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Young or senescent HUVECs were treated with either solvent or resveratrol (Resv, 10 µmol/L) for one hour. The cell lysates were then prepared and subjected to immunoblotting analysis of Akt-S473 (p-Akt), total Akt, S6-S235/S236 (p-S6), and total S6. Quantification of the signals is shown in the lower panels. n = 6, ***p<0.001 between indicated groups.
