Table 4.
Rescue of Top-GFP expression in hmgb1/2-double morphants by hmgb1/2 mRNA at 18-somite stage
| Normal expression | Significantly reduced expression | Expression at an intermediate level* | |
|---|---|---|---|
| Control MO (2 ng) | 104 (98.1%) |
2 (1.9%) |
0 |
|
hmgb1 MO (1 ng) + hmgb2 MO (1 ng) |
2 (3.4%) |
55 (93%) |
2 (3.4%) |
|
Hmgb1 mRNA (100 pg) + Hmgb2 mRNA (100 pg) |
24 (100%) |
0 (0%) |
0 (0%) |
|
hmgb1 MO (1 ng) + hmgb2 MO (1 ng) + Hmgb1 mRNA (100 pg)+ Hmgb2 mRNA (100 pg) |
9 (13.2%) |
33 (48.5%) |
26 (38.2%) |
*
Weaker than normal expression but clearly stronger than expression with severe downregulation